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Bioinformatics Service Report

IMMAGINA Biotechnology S.r.l.

07/03/2024

Thank you for choosing IMMAGINA Biotechnology for your analysis needs! This interactive report provides a comprehensive overview of your results, with easy-to-read graphs and charts that bring your data to life.

If you have any questions about the analyses, please don’t hesitate to contact us at

Workflow

1. Alignment Statistics

Table 1:

Percentage of reads uniquely and multi aligned to the transcriptome after removal of rRNA, tRNA and snRNA reads.

2. Riboseq QC

Read length

This plot shows the read length distribution respect to total reads. RPFs are generally between 30nt and 40nt length (depends on conformation).

Region & Frame

This plot shows the percentage of P-sites mapping to the 5′ UTR, coding sequence (CDS), and 3′ UTR corresponding to the three possible reading frames.

Heatmap (Norm. tot Reads)

This plot shows the percentage of P-sites corresponding to the three possible reading frames along the 5′ UTR, CDS, and 3′ UTR, stratified for read length. The percentage is normalized on the total number of reads.

3. Riboseq Global

Metaprofile

Metaprofile plot shows P Site positions for all transcripts around translation initiation sites (TISs) and translation termination sites (TTSs).

Covered Area

Coverage plot shows for every transcript (each column) the percentage of the total length covered at least 1 read.

Codon Usage

Codon Usage plot show the frequency of in-frame P-sites along the coding sequence associated to each codon, normalized for codon frequency in sequences.

4. Outputs

* Raw Data
* MultiQC html report
* BAM files
* Table with n° of reads for each transcript
* Table with n° of in-frame reads for each transcript
* Table with psite information for each read
* Plots