IMMAGINA’s proprietary RiboLace technology enables fast and selective extraction of ribosomes in active translation (both in vitro and in vivo) and purification of ribosome protected fragments (RPFs) for continuing with the ribosome profiling library preparation.
The technology combines a module for sample preparation, three different options for ribosome profiling library preparation/sequencing (RiboLace module 2, LACEseq and circAID-p-seq.) and a kit for gel extraction. The technology allows the user to perform ribosome profiling experiments with a short and simple workflow, no ultracentrifugation steps and low cell input requirements (>100.000 cells in active translation).
- Ribosomal profiling
- Translatome analysis