19 March 2024
Ribosome profiling identifies translating RNA, but sequencing struggles with abundant rRNA. Removing rRNA boosts relevant reads (80-90%). Different methods have advantages and limitatations. Choosing the best method depends on your experiment.
19 March 2024
3' modifications (phosphate, cyclic phosphate) on RNA (mRNA, rRNA, tRNA) are not dead ends but can be functional molecules in RNA metabolism & stress response. Standard RNA-seq misses them. tRNA fragments (tiRNAs) can be an example.
19 March 2024
Current methods miss details of active protein production. RiboLace isolates active ribosomes without tags, offering better data quality with less sample. It even captures disomes, potentially revealing new aspects of translation and disease links.
19 March 2024
Beyond RNA: Protein control is crucial for cells. New methods (ribosome profiling) reveal complex regulation of translation, including specialized ribosomes for specific protein production.
19 March 2024
Transcriptome poorly predicts protein levels. New methods (Polysome Profiling, Ribosome Profiling) study ribosome-mRNA interactions to decipher translational control of gene expression.
