26 March 2024
RiboLace, a novel method using a unique puromycin derivative molecule, specifically captures actively translating ribosomes, enabling detailed analysis of genome-wide translation activity at single-nucleotide resolution.
19 March 2024
Ribosome profiling identifies translating RNA, but sequencing struggles with abundant rRNA. Removing rRNA boosts relevant reads (80-90%). Different methods have advantages and limitatations. Choosing the best method depends on your experiment.
8 March 2024
Nano-tRNAseq, a new method using nanopores, allows for simultaneous quantification of tRNA abundance and modifications in a single experiment. This overcomes limitations of previous methods and reveals details of tRNA populations in yeast under stress.
8 March 2024
Ribo-Zero rRNA depletion kit discontinued. New alternatives unsuited for ribosome profiling. Consider traditional biotinylated oligos for accurate translation analysis.
7 March 2024
SMN protein, linked to spinal muscular atrophy (SMA), steers ribosomes on specific mRNAs affecting translation of key neuronal proteins. Loss of SMN disrupts this process, contributing to SMA.
1 March 2024
New R package (expressyouRcell) simplifies visualizing complex gene expression data across time, space, and single cells. It uses dynamic cell pictographs for clear communication of results, improving on traditional quantitative methods.
