LACEseq (12rxns)
LACEseq streamlines and simplifies library preparation for RiboSeq experiments thanks to:
- Selective Targeting: LACEseq leverages the phosphate group left behind by nucleases used to isolate ribosome protected fragments (RPFs). Standard methods remove this tag, but LACEseq uses it to specifically target RPFs for library inclusion.
- Efficient Ligation: A two-step ligation process ensures preferential uptake of RPFs while minimizing RNA contaminants.
- Optimized Linker: The LACEseq linker is designed for compatibility with downstream NGS sequencing and data analysis. It also includes Unique Molecular Identifiers (UMIs) to identify PCR duplicates.
- Multiplexing Ready: Illumina adapters and Unique Dual Indexes (iUDIs) for multiplexing are added after a PCR amplification step (iUDIs sold separately).
(Note: The LACEseq kit does not include iUDIs. Immagina offers four sets of iUDIs , sold separately.)
The LACEseq kit creates high-quality NGS libraries from small RNAs with 3' phosphate tags in just one day. Ideal for libraries made from Ribosome Protected Fragments (RPFs) isolated with RiboLace kits or other methods. Compatible with Illumina sequencing platforms.
Shipping information
Please note that LACEseq kit will be shipped in dry ice. The shipping is usually scheduled within 1 week from your order. We pride ourselves on rapid and thorough support to ensure your success with our new technology. Please contact us with any questions.
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