Go to homepage
€0.00*

AHARIBO


Technology

AHARIBOTM leverages L-azidohomoalanine (AHA)-mediated ribosome isolation to provide a rapid and cost-efficient two-step method to isolate the active translatome and its components: the full population of actively translated mRNAs, the attached translating ribosomes, and the nascent proteins.

AHARIBO provides simplified, faster, and more cost-efficient workflows than conventional polysome profiling or affinity purification-based techniques. Improved correlation between mRNA and protein levels can provide greater confidence in results. 





The protocol is based on a pulse incubation of cells with l-azidohomoalanine (AHA) followed by sBlock treatment to stabilize nascent peptides on the translating ribosomes. The newly synthesized AHA-labelled peptides are then used as tags for the separation of active ribosome complexes through chemical interactions with magnetic beads.

AHARIBO RNA and Protein System enables to perform RNA analysis such as qPCR or RNAseq on translating RNAs in parallel to de novo protein synthesis analysis.  

IMMAGINA Biotechnology s.r.l. exclusively owns and holds full IP rights for the AHARIBO technology.

AHARIBO provides simplified, faster, and more cost-efficient workflows than conventional polysome profiling or affinity purification-based techniques. Improved correlation between mRNA and protein levels can provide greater confidence in results. 

The protocol is based on a pulse incubation of cells with l-azidohomoalanine (AHA) followed by sBlock treatment to stabilize nascent peptides on the translating ribosomes.

The newly synthesized AHA-labelled peptides are then used as tags for the separation of active ribosome complexes through chemical interactions with magnetic beads. 

AHARIBO RNA and Protein System enables to perform RNA analysis such as qPCR or RNAseq on translating RNAs in parallel to de novo protein synthesis analysis.  

 IMMAGINA Biotechnology s.r.l. exclusively owns and holds full IP rights for the AHARIBO technology.

Applications

  • Translatome analysis
  • De novo proteome analysis
  • Proteotranscriptomic
  • Ribosome heterogeneity studies
  • lncRNA translation


This website uses cookies to ensure the best experience possible. More information...