Bioinformatics Service Report
IMMAGINA Biotechnology S.r.l.
04/03/2024
Thank you for choosing IMMAGINA Biotechnology for your analysis needs!
This interactive report provides a comprehensive overview of your
results, with easy-to-read graphs and charts that bring your data to
life.
If you have any questions about the analyses, please don’t hesitate to
contact us at info@immaginabiotech.com
1. Samples description
2. Results
Cells pellets were clarified for PBS removal and resuspended in 400 µl
of IMMAGINA polysome lysis buffer cells were lysed by passing them
through a G26 needle (~10 times). The nuclei were then pellet by
centrifugation at 20000 g for 5 min, the supernatant was transferred to
a new tube and left on ice for 20 min. A.U. obtained were measured at
Nanodrop and are enlisted in the following table.
3. A.U. Measured
4. Polysome Profile
Optional Opportunities
Polysome
profile fractions are collected and stored for 30 days from the report
delivery date. Upon request, we store fractions for a longer period of
time. We do offer RNA sequencing of single or pulled fractions
(polysomal and sub-polysomal). This allows you to identify highly and
low translated mRNAs to deeper understand gene expression and its
regulation in you experimental system.If interested, please contact
Sales and Service Team.
5. Translation Efficiency (TE) Calculation
Translation Efficiency (TE) is obtained as a ratio between the area
under the curve of polysomes and the sum between the 80S peak and the
polysomes areas, see reference figure below.
Representative polysomal profile. The area under the
monosome (80S) peak (pink) and the area under the polysomes peak (light
blue) were used to calculate the TE.
6. TE Results
Samples treated with Harringtonine show a significant decrease in TE
compared to control. (***P<0.001, Welch’s t-test)
