Bioinformatics Service Report

IMMAGINA Biotechnology S.r.l.

28/02/2024

Thank you for choosing IMMAGINA Biotechnology for your Nano-tRNAseq experiments! This report provides a comprehensive overview of your results, with easy-to-read graphs and charts that bring your data to life.

If you have any questions about the experiment, please don’t hesitate to contact us at info@immaginabiotech.com

1. Analysis of tRNA abundances

Scatter plots of tRNA abundances by aminoacid type across samples.

2. Analysis of putative tRNA modifications

Heatmap of the difference in summed basecalling errors between two samples.

3. Outputs

* Bulk fast5 sequencing files

* Demultiplexed fastq files

* Alignment BAM files

* Table with n° of uniquely mapped reads for each tRNA (counts.tsv). It can be used to reproduce the scatterplots.

* Table with n° of times a nucleotide was basecalled correctly or an error (mismatch,insertion or deletion) was made for each tRNA nucleotide in each sample (heatmap.base_count.tsv)

* Table with the sum of basecalling errors for each tRNA nucleotide divided by the depth of coverage for that position in each sample (heatmap.base_freq.tsv). It can be used to reproduce the heatmaps.